The response to the application of an allergen on the seed coat will be three (3) different types: negative, allergic, irritative.

• Subjective symptoms (pruritus, pain, lack of sensation) are random and should not consider only incidentally.

• A negative response is synonymous with lack of any reaction: the seed coat is of normal appearance. There is no detectable lesions.

• A positive response "in miniature" reproduces a clinical picture of eczema, with varying degrees of intensity. Allergic nature responses may extend beyond the territory of application of allergens, with fuzzy limits or, instead, remain perfectly delimited. The allergic reaction is generally spread evenly over the extent of the site, given the regular dispersal of the allergen in vaseline.

• A positive response of irritative nature can take different clinical aspects.